How to Prepare 5M NaCl in 50 mL
Preparing a 5M (molarity) solution of sodium chloride (NaCl) in a 50 mL volume is a common task in laboratories and scientific research. This concentration is often used for various applications, including cell culture, microbiology, and chemical reactions. In this article, we will guide you through the steps to prepare a 5M NaCl solution in 50 mL, ensuring accuracy and safety in the process.
Materials Needed
Before starting, gather the following materials:
– Sodium chloride (NaCl) powder or pellets
– Distilled water
– A 50 mL volumetric flask
– A balance or scale
– A graduated cylinder or pipette
– Safety equipment, such as gloves and goggles
Step 1: Calculate the Amount of NaCl Needed
To prepare a 5M NaCl solution, you need to know the molar mass of NaCl, which is approximately 58.44 g/mol. The formula for calculating the mass of NaCl needed is:
Mass (g) = Molarity (M) × Volume (L) × Molar Mass (g/mol)
In this case, you want to prepare 5M NaCl in 50 mL (0.050 L):
Mass (g) = 5 M × 0.050 L × 58.44 g/mol
Mass (g) = 1.472 g
Therefore, you will need 1.472 grams of NaCl to prepare the solution.
Step 2: Weigh the NaCl
Using a balance or scale, weigh out 1.472 grams of NaCl. Be sure to wear gloves and goggles to protect yourself from any potential contact with the chemical.
Step 3: Dissolve the NaCl in Distilled Water
Add the weighed NaCl to a 50 mL volumetric flask. Then, add a small amount of distilled water to the flask and swirl gently to dissolve the NaCl. It may take a few minutes for the NaCl to completely dissolve.
Step 4: Fill the Flask to the Mark
Once the NaCl is dissolved, continue adding distilled water to the flask until the solution reaches the 50 mL mark. Be careful not to exceed the mark, as this could result in an incorrect concentration.
Step 5: Mix the Solution
After filling the flask to the mark, invert the flask several times to ensure that the solution is well-mixed. This step is crucial for achieving a uniform concentration throughout the solution.
Step 6: Safety Precautions
When handling NaCl, always follow proper safety precautions. Wear gloves and goggles to protect yourself from any potential contact with the chemical. Also, be sure to work in a well-ventilated area to avoid inhaling any dust or fumes.
Conclusion
Preparing a 5M NaCl solution in 50 mL is a straightforward process that requires careful measurement and mixing. By following these steps and using the appropriate safety equipment, you can ensure that your solution is accurate and safe for use in your experiments or applications.
