How to Prepare 0.1 M EDTA Solution
EDTA (Ethylenediaminetetraacetic acid) is a versatile chelating agent commonly used in various scientific applications, including water treatment, pharmaceuticals, and analytical chemistry. One of the most common concentrations used for EDTA solutions is 0.1 M (molar). Preparing a 0.1 M EDTA solution is a straightforward process, but it requires precision and attention to detail to ensure the desired concentration is achieved. In this article, we will guide you through the steps to prepare a 0.1 M EDTA solution.
Materials Needed
Before you begin, gather the following materials:
– EDTA powder (anhydrous)
– Distilled water
– Weighing scale
– Measuring cylinder
– Beaker
– Stirrer
– Safety equipment (gloves, lab coat, and safety goggles)
Step 1: Calculate the Mass of EDTA Needed
To prepare a 0.1 M EDTA solution, you need to calculate the mass of EDTA powder required. The molecular weight of EDTA is 292.24 g/mol. To make 1 liter of 0.1 M EDTA solution, you will need:
Mass of EDTA = (0.1 M) x (292.24 g/mol) x (1 L) = 29.224 g
Step 2: Weigh the EDTA Powder
Using a weighing scale, accurately measure out 29.224 g of anhydrous EDTA powder. It is crucial to ensure that the scale is properly calibrated and that you are using a container that is clean and dry to avoid contamination.
Step 3: Dissolve EDTA in Distilled Water
Add the weighed EDTA powder to a beaker containing approximately 100 mL of distilled water. Stir the mixture gently to dissolve the EDTA. If the powder does not dissolve completely, you can gently heat the solution using a hot plate or water bath, but be cautious not to overheat.
Step 4: Transfer the Solution to a Measuring Cylinder
Once the EDTA is completely dissolved, transfer the solution to a 1-liter measuring cylinder. Be sure to use a funnel to avoid spilling the solution.
Step 5: Add Distilled Water to Reach the Mark
Fill the measuring cylinder with distilled water up to the 1-liter mark. Swirl the cylinder gently to ensure that the solution is well-mixed.
Step 6: Stir the Solution
Stir the solution thoroughly to ensure that the EDTA is evenly distributed throughout the water. Allow the solution to cool to room temperature before using it.
Step 7: Store the Solution
Transfer the 0.1 M EDTA solution to a clean, labeled bottle and store it in a cool, dry place. EDTA solutions can be stored for several months without losing their effectiveness.
By following these steps, you can successfully prepare a 0.1 M EDTA solution. Always remember to work safely and accurately, as EDTA can be harmful if ingested or inhaled.
